Seminar 11/06/2009 Laser Ablation Electrospray Ionization (LAESI) for High Throughput and Imaging Mass Spectrometry

Department of Chemical Engineering
West Virginia University

Seminar

Laser Ablation Electrospray Ionization (LAESI) for High Throughput and Imaging Mass Spectrometry



Matthew Powell
Protea Biosciences, Inc.
Morgantown, WV

ABSTRACT

Atmospheric pressure mass spectrometry is a rapidly expanding field that offers advantages in the ability to study biological systems in their native condition, simplified sample preparation and high throughput. Among these techniques, the spatial organization and characterization of biomolecules and xenobiotics in biological tissues by imaging mass spectrometry has been a point of emphasis for both academic and commercial research in recent years. Techniques including desorption electrospray ionization (DESI), matrix-assisted laser desorption ionization (MALDI), and atmospheric pressure IR matrix-assisted laser desorption ionization (AP IR-MALDI) have been used with varying degrees of success for different types of samples based on the strengths and weaknesses of the individual methods. Using native water content as the “matrix’ for energy absorbance by a mid-IR laser, laser ablation electrospray ionization (LAESI) represents the next evolution of imaging mass spectrometry. Biological samples can be directly analyzed without the need for elaborate sample prep, and a wide variety of classes, sizes, and dynamic ranges of biomolecules can be characterized by LAESI mass spectrometry to produce sophisticated 2D and 3D molecular imaging.

Friday, November 6, 2009 9:30am - 10:45am
Room 401, Engineering Sciences Building
Refreshments served at 9:15 am

Questions or Directions call: 304-293-9344.

11/06/2009